Hanging Drop Mount

Ingredients

• toothpick
• pipette
• coverslip
• well-slide
• petroleum jelly
• drop of stagnant water
• microscope

Procedure 

1. Place a coverslip on a clean, flat surface. Use a toothpick to spread a small amount of petroleum jelly around all the edges of the coverslip. Use enough to form a seal between the coverslip and the slide.
2. Using a pipette, place a drop of the  stagnant water in the center of the coverslip.
3. Invert a well slide (well side down). Center the well over the coverslip. Use just enough pressure to make sure the coverslip seals to the slide (Don't press to hard or the jelly will ooze into the specimen area).
4. Invert the assembly so the coverslip is on top. The drop should be suspended in the well from the coverslip).
5. Observe the slide under a microscope.

Observations

In the drop I found a cluster of organisms. I saw cells, long cells and some moving tiny organisms.

Long cells 100x

Cluster at  45x

More long cells 100x

Four cells 100x

Five different cells 100x